Functional changes in the retina during and after acute intraocular pressure elevation in mice

Abstract

Purpose. To examine retinal function using the full-field elec-troretinogram (ERG) during and after acute intraocular pressure (IOP) elevation in wild-type mice. Methods. IOP was elevated by anterior chamber cannulation in wild-type C57/BL6 mice. The pressure-function relationship was determined by IOP elevation in steps from baseline to 80 mm Hg. The rate of functional recovery was assessed for 60 minutes after an IOP spike of 50 mm Hg for 30 minutes. During and immediately after IOP elevation, scotopic ERG signals were recorded in response to dim and bright flashes (-4.54, -2.23, and 0.34 log cd • s • m-2) and analyzed for photoreceptoral (a-wave), ON-bipolar (b-wave), oscillatory potentia..